PD-ECGF Rekombinanter Antikörper

PD-ECGF Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251581B2

Kat-Nr. : 86618-3-RR

Synonyme

TYMP, EC:2.4.2.4, ECGF1, Gliostatin, Platelet-derived endothelial cell growth factor

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  WB analysis using 86618-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86618-3-RR (PD-ECGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86618-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86618-3-RR (PD-ECGF antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86618-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86618-3-RR against Human PD-ECGF were performed. The affinity constant is 1.64 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, THP-1-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86618-3-RR bindet in WB, ELISA PD-ECGF und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-ECGF fusion protein Ag3052
• Vollständiger Name: thymidine phosphorylase
• Berechnetes Molekulargewicht: 482 aa, 50 kDa
• Beobachtetes Molekulargewicht: 50 kDa
• GenBank-Zugangsnummer: BC018160
• Gene symbol: Thymidine phosphorylase
• Gene ID (NCBI): 1890
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet derived endothelial cell growth factor (PD-ECGF), also known as thymidine phosphorylase (TP), induces migration and angiogenesis in endothelial and tumor cells, and was upregulated in various malignancies compared to that in normal tissues. Interestingly, PD-ECGF has dual effect on tumor development and chemotherapy. It could stimulate cancer cell migration and proliferation. On the other hand, some chemotherapeutic agents (5-fluorouracil, capecitabine, etc.) were converted to their active forms through TP enzymes.