PD-L1/CD274 Rekombinanter Antikörper

PD-L1/CD274 Rekombinant Antikörper für WB, IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

240993C9

Kat-Nr. : 83906-6-RR

Synonyme

Cd274, PD-L1, 240993C9, B7 H1, PD L1

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  WB analysis using 83906-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83906-6-RR (PD-L1/CD274 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse spleen using 83906-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse spleen using 83906-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 83906-6-RR (PD-L1/B7-H1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human placenta using 83906-6-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PD-L1/CD274 antibody (83906-6-RR, Clone: 240993C9 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83906-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83906-6-RR against Mouse PD-L1/B7-H1 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mausherzgewebe, Rattenherzgewebe, Rattennierengewebe
• Erfolgreiche Detektion in IHC: Mausmilzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83906-6-RR bindet in WB, IHC, IF-P, ELISA PD-L1/CD274 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-L1/CD274 fusion protein Eg0986
• Vollständiger Name: CD274 antigen
• Berechnetes Molekulargewicht: 33 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: NM_021893.3
• Gene symbol: PD-L1
• Gene ID (NCBI): 60533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.