PD-L1/CD274 Monoklonaler Antikörper

PD-L1/CD274 Monoklonal Antikörper für FC

Wirt / Isotyp

Maus / IgG2b, kappa

Getestete Reaktivität

human

Anwendung

FC

Konjugation

FITC Plus Fluorescent Dye

CloneNo.

29E.2A3

Kat-Nr. : FITC-65081

Synonyme

CD274, PD-L1, PD L1, hPD-L1, B7-H1

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  FC experiment of human PBMCs using FITC-65081

  1x10^6 PHA treated (3 days) human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (red) or FITC Plus Mouse IgG2b Isotype Control (blue). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (black dashed). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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  FC experiment of human PBMCs using FITC-65081

  1x10^6 PHA treated (3 days) human PBMCs were surface stained with CL750 Anti-Human CD3 and 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (right) or FITC Plus Mouse IgG2b Isotype Control (left). 1x10^6 untreated human PBMCs were surface stained with 5 ul FITC Plus Anti-Human PD-L1 (B7-H1) (FITC-65081, Clone:29E.2A3) (center). Cells were not fixed. Cells were treated with FC Receptor Block prior to staining. Lymphocytes were gated.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 μl per 10^6 cells in a 100 µl suspension or 5 μl per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

FITC-65081 bindet in FC PD-L1/CD274 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG2b, kappa
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: Humanes Volllängen-PD-L1
• Vollständiger Name: CD274 molecule
• GenBank-Zugangsnummer: BC069381
• Gene symbol: PD-L1
• Gene ID (NCBI): 29126
• Konjugation: FITC Plus Fluorescent Dye
• Excitation/Emission maxima wavelengths: 495 nm / 524 nm
• Form: Liquid
• Reinigungsmethode: Affinitätsreinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.