PD-L1/CD274 (C-terminal) Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PD-L1/CD274 (C-terminal) Polyklonal Antikörper für WB, IHC, IF-P, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, IP, CoIP, ChIP, ELISA

Konjugation

Unkonjugiert

Publikationen(114)

Kat-Nr. : 28076-1-AP

Synonyme

CD274, PD-L1, B7 H1, B7-H1, hPD-L1

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  WB analysis using 28076-1-AP

  Untreated and PNGase F-treated lysates of human placenta were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808).

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  WB analysis using 28076-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28076-1-AP

  Lysates of untreated and IFN gamma treated A549 cells were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 28076-1-AP

  WB result of PD-L1/CD274 (C-terminal) antibody (28076-1-AP; 1:300; incubated at room temperature for 1.5 hours) with sh-Control and sh-PD-L1/CD274 (C-terminal) transfected THP-1 cells.

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  WB analysis using 28076-1-AP

  MCF-7 cells (PD-L1-), MDA-MB-231 cells (PD-L1+), and human placenta tissue were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 28076-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 28076-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MDA-MB-231 using 28076-1-AP

  IP result of anti-PD-L1/CD274 (C-terminal) (IP:28076-1-AP, 4ug; Detection:28076-1-AP 1:3000) with MDA-MB-231 cells lysate 920 ug.

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  IHC staining of human tonsillitis using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28076-1-AP (PD-L1/CD274 antibody) at dilution of 1:800 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 28076-1-AP (PD-L1/CD274 antibody) at dilution of 1:800 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28076-1-AP (PD-L1/CD274 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 28076-1-AP (PD-L1/CD274 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cervical cancer using 28076-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 28076-1-AP (PD-L1/CD274 (C-terminal) antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 28076-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PD-L1/CD274 (C-terminal) antibody (28076-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 28076-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using PD-L1/CD274 (C-terminal) antibody (28076-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite®594 CD3 antibody (CL594-60181, Clone: 3F3A1, red).

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  IF Staining of human placenta using 28076-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PD-L1/CD274 (C-terminal) antibody (28076-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: mit IFN-gamma behandelte A549-Zellen, humanes Plazenta-Gewebe, MDA-MB-231-Zellen, Mausherzgewebe, Rattenherzgewebe, THP-1-Zellen
• Erfolgreiche IP: MDA-MB-231-Zellen
• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Mammakarzinomgewebe, humanes Zervixkarzinomgewebe, humanes Lungenkarzinomgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 73 publications below
• IHC: See 44 publications below
• IF: See 24 publications below
• IP: See 3 publications below
• ELISA: See 1 publications below
• CoIP: See 1 publications below
• ChIP: See 1 publications below

Produktinformation

28076-1-AP bindet in WB, IHC, IF-P, IP, CoIP, ChIP, ELISA PD-L1/CD274 (C-terminal) und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PD-L1/CD274 (C-terminal) fusion protein Ag27557
• Vollständiger Name: CD274 molecule
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC074984
• Gene symbol: PD-L1
• Gene ID (NCBI): 29126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis. 

What is the molecular weight of PD-L1? 

Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa). 

What are the isoforms of PD-L1? 

According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions. 

What is the subcellular localization and tissue specificity of PD-L1? 

It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues. 

What is the function of PD-L1 in immune responses? 

PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681). 

How can PD-L1's implication in cancer be used as a drug target? 

In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).

Publikationen

Species	Application	Title
mouse	WB	Mol Cancer; Targeting PERP promotes anti-tumor immunity in HNSCC by regulating tumor immune microenvironment and metabolic homeostasis; Authors - Xueying Wang
mouse	WB,IP,IF	Cell Metab; Dual impacts of serine/glycine-free diet in enhancing antitumor immunity and promoting evasion via PD-L1 lactylation; Authors - Huan Tong
mouse	IHC	ACS Cent Sci; Allosteric Regulation of IGF2BP1 as a Novel Strategy for the Activation of Tumor Immune Microenvironment; Authors - Yang Liu
human	IF	Nat Commun; A protease-cleavable liposome for co-delivery of anti-PD-L1 and doxorubicin for colon cancer therapy in mice; Authors - Yixuan Liu
human	IF	Cell Rep Med; Benzosceptrin C induces lysosomal degradation of PD-L1 and promotes antitumor immunity by targeting DHHC3; Authors - Qun Wang
mouse,human	WB,IHC	Sci Adv; Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation; Authors - Wei Xiang