PD-L1/CD274 Rekombinanter Antikörper

PD-L1/CD274 Rekombinant Antikörper für FC

Wirt / Isotyp

Maus / IgG2a

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

10F.9G2

Kat-Nr. : 65685-1-MR

Synonyme

Cd274, PD-L1, B7 H1, PD L1, Pdcd1l1

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  FC experiment of mouse splenocytes using 65685-1-MR

  1x10^6 untreated or anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone:10F.9G2) and PE-conjugated Goat Anti-Mouse IgG. Cells were co-stained with APC Anti-Mouse CD3. Cells were not fixed.

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  FC experiment of mouse splenocytes using 65685-1-MR

  1x10^6 anti-CD3/CD28 treated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone: 10F.9G2) (red) or Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue) and PE-conjugated Goat Anti-Mouse IgG. 1x10^6 untreated mouse splenocytes were surface stained with 0.25 ug Anti-Mouse PD-L1/CD274 (10F.9G2) Mouse IgG2a RecAb (65685-1-MR, Clone:10F.9G2) (black, dashed) and PE-conjugated Goat Anti-Mouse IgG. Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: Anti-CD3/CD28 treated mouse splenocytes

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

65685-1-MR bindet in FC PD-L1/CD274 und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Maus / IgG2a
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: k.A.
• Vollständiger Name: CD274 antigen
• GenBank-Zugangsnummer: BC066841
• Gene symbol: PD-L1
• Gene ID (NCBI): 60533
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2-8°C. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.