PD-L1/CD274 Monoklonaler Antikörper

• Featured Product
• KD/KO Validated

PD-L1/CD274 Monoklonal Antikörper für WB, IHC, IF/ICC, IF-P, ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

2B11D11

Kat-Nr. : 66248-1-PBS

Synonyme

CD274, PD-L1, 2B11D11, B7 H1, B7-H1

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  WB analysis of human placenta using 66248-1-Ig (same clone as 66248-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis using 66248-1-Ig (same clone as 66248-1-PBS)

  Untreated and IFN gamma treated A375 cells and A549 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis of human skeletal muscle using 66248-1-Ig (same clone as 66248-1-PBS)

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis of RAW 264.7 using 66248-1-Ig (same clone as 66248-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  WB analysis of human placenta using 66248-1-Ig (same clone as 66248-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis of pig lung using 66248-1-Ig (same clone as 66248-1-PBS)

  pig lung tissue were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 cells using 66248-1-Ig (same clone as 66248-1-PBS)

  WB result of PD-L1 antibody (66248-1-Ig, 1:1000 and 1:2000) with si-Control and si-PDL1 transfected HepG2 cells. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66248-1-Ig (same clone as 66248-1-PBS)

  HepG2, K-562, HSC-T6, and Raw 264.7 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  WB analysis of THP-1 using 66248-1-Ig (same clone as 66248-1-PBS)

  Untreated and PNGase F-treated lysates of THP-1 cells and HepG2 cells were subjected to SDS PAGE followed by western blot with 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. PNGase F was obtained from Atagenix (cat.NO. ata808). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  IHC staining of human tonsillitis using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:10000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human lung cancer using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:20000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). Slide was treated with PNGase F before staining. This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66248-1-Ig (PD-L1/CD274 Antibody) at dilution of 1:500 (under 10x lens). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66248-1-Ig (PD-L1/CD274 Antibody) at dilution of 1:500 (under 40x lens). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66248-1-Ig (PD-L1/CD274 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human placenta using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using PD-L1/CD274 antibody (66248-1-Ig, Clone: 2B11D11 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66248-1-Ig (same clone as 66248-1-PBS)

  Immunofluorescent analysis of (10% Formaldehyde ) fixed HeLa cells using 66248-1-Ig(PD-L1/CD274 antibody) at dilution of 1:300 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66248-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66248-1-PBS bindet in WB, IHC, IF/ICC, IF-P, ELISA PD-L1/CD274 und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PD-L1/CD274 fusion protein Ag12443
• Vollständiger Name: CD274 molecule
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa, 33 kDa
• GenBank-Zugangsnummer: BC074984
• Gene symbol: PD-L1
• Gene ID (NCBI): 29126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis. 

What is the molecular weight of PD-L1? 

Depending on the isoform, the calculated molecular weight of the protein varies between 20 and 33 kDa (176-290 aa). 

What are the isoforms of PD-L1? 

According to NCBI, three different isoforms have been identified. There are significant differences in the untranslated and protein coding regions. 

What is the subcellular localization and tissue specificity of PD-L1? 

It is predicted to localize in the plasma membrane of various cell types, with a particularly high expression in placental trophoblast and subsets of immune cells. High levels of PD-L1 protein have also been detected in lung and colon tissues. 

What is the function of PD-L1 in immune responses? 

PD-L1 is critical for the induction and maintenance of immune self-tolerance during infection or inflammation in normal tissues. The interaction of PD-L1 and its receptors is responsible for preventing auto-immune phenotypes and balancing the overall immune response in situations such as pregnancy or tissue allografts. The interaction between PD-L1 and PD-1 or B7.1 starts an inhibitory signaling cascade, which results in the decreased proliferation of antigen-specific T-cells and increased survival of regulatory T-cells (PMID: 15240681). 

How can PD-L1's implication in cancer be used as a drug target? 

In certain tumors, high expression of PD-L1 serves as a stop-sign to inhibit the recognition of cancer cells by T-cells (PMID: 23087408). The interaction between PD-L1 and its receptors (PD1 and B7.1) is a mechanism for the tumor to evade the host immune response (PMID: 29357948). Several mAbs have been developed to target that interaction and thus prevent the inactivation of cytotoxic T-cells by the tumor (PMIDs: 23890059, 18173375).