PD-L1/CD274 Rekombinanter Antikörper

PD-L1/CD274 Rekombinant Antikörper für IHC, IF-P, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IHC, IF-P, ELISA

Konjugation

Unkonjugiert

CloneNo.

3G4

Kat-Nr. : 82719-13-RR

Synonyme

CD274, PD-L1, 3G4, B7 H1, B7-H1

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  IHC staining of human tonsillitis using 82719-13-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 82719-13-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 82719-13-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 82719-13-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:414 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 82719-13-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using PD-L1/CD274 antibody (82719-13-RR, Clone: 3G4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 82719-13-RR

  Biolayer interferometry (BLl) kinetic assays of 82719-13-RR against Human PD-L1/CD274 were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF-P: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:200-1:800
• Immunfluoreszenz (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

82719-13-RR bindet in IHC, IF-P, ELISA PD-L1/CD274 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-L1/CD274 fusion protein Ag12432
• Vollständiger Name: CD274 molecule
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• GenBank-Zugangsnummer: BC074984
• Gene symbol: PD-L1
• Gene ID (NCBI): 29126
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, it is predicted to be 27-30 kDa after signal peptide cleavage (PMID: 25609200; 17076679). The apparent molecular weight has also been reported as 45-70 kDa, major glycosylated form of 45-50 kDa and multiple post-translational modifications form of 65-70 kDa (PMID: 18760278; 16493058).