PD-L1/CD274 Rekombinanter Antikörper

PD-L1/CD274 Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

CoraLite®594 Fluorescent Dye

CloneNo.

240721H8

Kat-Nr. : CL594-98062

Synonyme

CD274, PD-L1, 240721H8, B7 H1, B7-H1

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  FC experiment of human PBMCs using CL594-98062

  1x10^6 untreated or PHA-treated human PBMCs were surface stained with APC Anti-Human CD3 and 5 ul CoraLite®594 Anti-Human PD-L1/CD274 Rabbit RecAb (CL594-98062, Clone: 240721H8). Cells were not fixed.

• 

  FC experiment of human PBMCs using CL594-98062

  1x10^6 PHA-treated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human PD-L1/CD274 Rabbit RecAb (CL594-98062, Clone: 240721H8) (red) or CoraLite®594 Rabbit IgG Isotype Control RecAb (CL594-98136, Clone: 240953C9) (blue). Untreated human PBMCs were surface stained with 5 ul CoraLite®594 Anti-Human PD-L1/CD274 Rabbit RecAb (CL594-98062, Clone: 240721H8) (black, dashed). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: PHA treated human PBMCs

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been pre-titrated and tested for flow cytometric analysis. The suggested use of this reagent is 5 ul per 10^6 cells in a 100 µl suspension or 5 ul per 100 µl of whole blood.
Sample-dependent, check data in validation data gallery

Produktinformation

CL594-98062 bindet in FC PD-L1/CD274 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PD-L1/CD274 fusion protein Eg1114
• Vollständiger Name: CD274 molecule
• Berechnetes Molekulargewicht: 290 aa, 33 kDa
• GenBank-Zugangsnummer: BC074984
• Gene symbol: PD-L1
• Gene ID (NCBI): 29126
• Konjugation: CoraLite®594 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 588 nm / 604 nm
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide and 0.5% BSA
• Lagerungsbedingungen: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Hintergrundinformationen

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner.