PD-L2/CD273 Polyklonaler Antikörper

PD-L2/CD273 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 27406-1-PBS

Synonyme

PDCD1LG2/PD-L2, PDCD1LG2, PD-L2, B7 DC, B7DC

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  WB analysis using 27406-1-AP (same clone as 27406-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 27406-1-AP (PDCD1LG2/PD-L2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

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  IHC staining of rat heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 27406-1-AP (same clone as 27406-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 27406-1-AP (PD-L2/CD273 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

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  IF Staining of NIH/3T3 using 27406-1-AP (same clone as 27406-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using PDCD1LG2/PD-L2 antibody (27406-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 27406-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

27406-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PD-L2/CD273 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PD-L2/CD273 fusion protein Ag26384
• Vollständiger Name: programmed cell death 1 ligand 2
• Berechnetes Molekulargewicht: 273 aa, 31 kDa
• Beobachtetes Molekulargewicht: 45 kDa
• GenBank-Zugangsnummer: BC074766
• Gene symbol: PD-L2
• Gene ID (NCBI): 80380
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 ligand 2 (PDCD1LG2), also known as PD-L2, CD273 and B7-DC, is an immune checkpoint receptor ligand which plays a role in negative regulation of the adaptive immune response. PDCD1LG2/PD-L2 protein is expressed mainly by dendritic cells, macrophages, and tumor cells, and down-regulates the effector functions of T cells via PD-1/PD-L2 axis in the tumor microenvironment (PMID: 30275216). The endogenous PDCD1LG2/PD-L2 protein displays a molecular weight of ∼45 kDa(glycosylated form), and a minor band at 35 kDa (non-glycosylated form) in western blot (PMID: 28811964, PMID: 34697216).