PDAP1 Polyklonaler Antikörper

PDAP1 Polyklonal Antikörper für WB, IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(4)

Kat-Nr. : 15081-1-AP

Synonyme

PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28

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  WB analysis of MCF-7 using 15081-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15081-1-AP (PDAP1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 15081-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15081-1-AP (PDAP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of MCF-7 using 15081-1-AP

  IP result of anti-PDAP1 (IP:15081-1-AP, 4ug; Detection:15081-1-AP 1:1000) with MCF-7 cells lysate 2400ug.

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  IHC staining of human breast cancer using 15081-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15081-1-AP (PDAP1 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 15081-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15081-1-AP (PDAP1 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MCF-7 using 15081-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PDAP1 antibody (15081-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MCF7 cells, MCF-7-Zellen
• Erfolgreiche IP: MCF-7-Zellen
• Erfolgreiche Detektion in IHC: humanes Mammakarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: MCF-7-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Produktinformation

15081-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PDAP1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDAP1 fusion protein Ag7155
• Vollständiger Name: PDGFA associated protein 1
• Berechnetes Molekulargewicht: 21 kDa
• Beobachtetes Molekulargewicht: 28 kDa
• GenBank-Zugangsnummer: BC000684
• Gene symbol: PDAP1
• Gene ID (NCBI): 11333
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	IHC	Proteomics Clin Appl; SILAC-based quantitative proteomic analysis of gastric cancer secretome.; Authors - Marimuthu Arivusudar A
human	WB	Cancer Sci; Pancreatic ductal adenocarcinoma cells regulated the gemcitabine-resistance function of CAFs by LINC00460; Authors - Xiao‐Xu Zhu
human		Heliyon; Single-cell RNA-Seq reveals the heterogeneity of fibroblasts within the tympanojugular paraganglioma microenvironment; Authors - Shengming Wang
human,mouse	WB,CoIP	Sci Adv; Hepatovirus translation requires PDGFA-associated protein 1, an eIF4E-binding protein regulating endoplasmic reticulum stress responses; Authors - Takayoshi Shirasaki