PD-1/CD279 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PD-1/CD279 Polyklonal Antikörper für IF, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

IP, IHC, IF, FC, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen

71

Kat-Nr. : 18106-1-AP

Synonyme

CD279, hPD 1, hPD l, PD 1, PD1, PD-1, PD-1/CD279, PDCD1, programmed cell death 1, Protein PD 1, SLEB2

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Galerie der Validierungsdaten

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  IHC staining of human tonsillitis using 18106-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18106-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 18106-1-AP

  Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue slide using 18106-1-AP (PD-1/CD279 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 18106-1-AP

  Immunohistochemical analysis of paraffin-embedded human angioimmunoblastic T-cell lymphoma tissue slide using 18106-1-AP (PD-1/CD279 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of human tonsillitis using 18106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

• 

  IF Staining of human tonsillitis using 18106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 rabbit polyclonal antibody) at dilution of 1:100 and 60271-1-Ig (CD20 mouse monoclonal antibody) at dilution of 1:200, further stained with CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) for 18106-1-AP, and CoraLite594-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) for 60271-1-Ig.

• 

  IF Staining of human tonsillitis using 18106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human tonsillitis using 18106-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using 18106-1-AP (PD-1/CD279 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in IHC: humanes Tonsillitisgewebe, humanes Lymphomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: humanes Tonsillitisgewebe

Empfohlene Verdünnung

• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• IHC: See 36 publications below
• IF: See 17 publications below
• IP: See 1 publications below
• FC: See 2 publications below
• CoIP: See 1 publications below

Produktinformation

18106-1-AP bindet in IP, IHC, IF, FC, CoIP, ELISA PD-1/CD279 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PD-1/CD279 fusion protein Ag12470
• Vollständiger Name: programmed cell death 1
• Berechnetes Molekulargewicht: 288 aa, 32 kDa
• GenBank-Zugangsnummer: BC074740
• Gene symbol: PDCD1
• Gene ID (NCBI): 5133
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).

Publikationen

Species	Application	Title
human,mouse	IP	Nat Commun; ERK and USP5 govern PD-1 homeostasis via deubiquitination to modulate tumor immunotherapy; Authors - Xiangling Xiao
mouse		Sci Adv; Promoting the activation of T cells with glycopolymer-modified dendritic cells by enhancing cell interactions.; Authors - Liyin Yu
human	IHC	J Immunother Cancer; Helicobacter pylori infection disturbs the tumor immune microenvironment and is associated with a discrepant prognosis in gastric de novo diffuse large B-cell lymphoma.; Authors - Yuwei Deng
human,mouse	IHC,IF	Mol Ther; PD-1/PD-L1 blockade is a potent adjuvant in treatment of Staphylococcus aureus osteomyelitis in mice; Authors - Kaiqun Li
human	IHC	Brief Bioinform; Machine learning-based tumor-infiltrating immune cell-associated lncRNAs for predicting prognosis and immunotherapy response in patients with glioblastoma; Authors - Hao Zhang
human,mouse		Mol Ther; Efficient Gene Silencing by Adenine Base Editor-Mediated Start Codon Mutation.; Authors - Xinjie Wang

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