PDCD2 Polyklonaler Antikörper

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• KD/KO Validated

PDCD2 Polyklonal Antikörper für IHC, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Zebrafisch

Anwendung

WB, IP, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

7

Kat-Nr. : 10725-1-AP

Synonyme

PDCD2, programmed cell death 2, RP8, Zinc finger protein Rp 8, ZMYND7

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Galerie der Validierungsdaten

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  WB analysis of HeLa using 10725-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 10725-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of H7ES using 10725-1-AP

  H7ES cells were subjected to SDS PAGE followed by western blot with 10725-1-AP (PDCD2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10725-1-AP

  IP Result of anti-PDCD2 (IP:10725-1-AP, 4ug; Detection:10725-1-AP 1:500) with HeLa cells lysate 2800ug.

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  IHC staining of human ovary tumor using 10725-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 10725-1-AP (PDCD2 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, H7ES-Zellen, Mauslebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Ovarialkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:1000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 3 publications below

Produktinformation

10725-1-AP bindet in WB, IP, IHC, ELISA PDCD2 und zeigt Reaktivität mit human, Maus, Zebrafisch

• Getestete Reaktivität: human, Maus, Zebrafisch
• In Publikationen genannte Reaktivität: human, Maus, Zebrafisch
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDCD2 fusion protein Ag1139
• Vollständiger Name: programmed cell death 2
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 37-39 kDa, 45 kDa
• GenBank-Zugangsnummer: BC008378
• Gene symbol: PDCD2
• Gene ID (NCBI): 5134
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed Cell Death 2 (PDCD2) is widely expressed in embryonic and adult tissues but with undefined function yet. Recent observation uncovered its role in hematopoietic stem cell differentiation during development. Catalog# 10725-1-AP is a rabbit polyclonal antibody raised against full-length PDCD2 of human origin.

Publikationen

Species	Application	Title
human	WB	Mol Cell Proteomics; Quantitative proteomic analysis of cellular protein modulation upon inhibition of the NEDD8-activating enzyme by MLN4924.; Authors - Hua Liao
human		Pharmacol Res; Chip-DSF: A rapid screening strategy for drug protein targets.; Authors - Zhao Cui
		Br J Haematol; MicroRNA 200a inhibits erythroid differentiation by targeting PDCD4 and THRB.; Authors - Yanming Li
mouse	IHC	Sci Rep; Single-cell RNA-seq reveals distinct injury responses in different types of DRG sensory neurons.; Authors - Ganlu Hu
human, mouse, zebrafish	IHC	Stem Cells Dev; PDCD2 controls hematopoietic stem cell differentiation during development.; Authors - Kramer Joseph J
mouse	WB	Dev Biol; PDCD2 is essential for inner cell mass development and embryonic stem cell maintenance.; Authors - Mu Weipeng W ; KO Validated