PDCD4 Polyklonaler Antikörper

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• KD/KO Validated

PDCD4 Polyklonal Antikörper für WB, IHC, FC (Intra), IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, FC (Intra), IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12587-1-PBS

Synonyme

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  WB analysis using 12587-1-AP (same clone as 12587-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK293 cells using 12587-1-AP (same clone as 12587-1-PBS)

  WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12587-1-AP (same clone as 12587-1-PBS)

  IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12587-1-AP (same clone as 12587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12587-1-PBS bindet in WB, IHC, FC (Intra), IP, Indirect ELISA PDCD4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDCD4 fusion protein Ag3288
• Vollständiger Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 54-64 kDa
• GenBank-Zugangsnummer: BC026104
• Gene symbol: PDCD4
• Gene ID (NCBI): 27250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.