PDCD4 Monoklonaler Antikörper

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• KD/KO Validated

PDCD4 Monoklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1F1A11

Kat-Nr. : 66100-1-PBS

Synonyme

H731, Nuclear antigen H731 like, PDCD4, Protein 197/15a

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  WB analysis of HEK293 cells using 66100-1-Ig (same clone as 66100-1-PBS)

  WB result of PDCD4 antibody (66100-1-Ig, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 66100-1-Ig (same clone as 66100-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66100-1-Ig (same clone as 66100-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 66100-1-Ig (same clone as 66100-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66100-1-Ig (PDCD4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66100-1-Ig (same clone as 66100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66100-1-Ig (PDCD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66100-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66100-1-PBS bindet in WB, IHC, Indirect ELISA PDCD4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDCD4 fusion protein Ag19239
• Vollständiger Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Berechnetes Molekulargewicht: 469 aa, 52 kDa
• Beobachtetes Molekulargewicht: 56 kDa
• GenBank-Zugangsnummer: BC026104
• Gene symbol: PDCD4
• Gene ID (NCBI): 27250
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A.