PDCD6/ALG2 Rekombinanter Antikörper

PDCD6/ALG2 Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

250087B4

Kat-Nr. : 85792-5-RR

Synonyme

ALG2, PDCD6, ALG 2, ALG-2, Apoptosis-linked gene 2 protein homolog

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  WB analysis using 85792-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 85792-5-RR

  HeLa cells were subjected to SDS PAGE followed by western blot with 85792-5-RR (PDCD6/ALG2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 85792-5-RR

  Biolayer interferometry (BLl) kinetic assays of 85792-5-RR against Human PDCD6/ALG2 were performed. The affinity constant is 5.93 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HepG2-Zellen, HEK-293-Zellen, HeLa-Zellen, K-562-Zellen, NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85792-5-RR bindet in WB, ELISA PDCD6/ALG2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDCD6/ALG2 fusion protein Ag2949
• Vollständiger Name: programmed cell death 6
• Berechnetes Molekulargewicht: 191 aa, 22 kDa
• Beobachtetes Molekulargewicht: 15-22 kDa
• GenBank-Zugangsnummer: BC012384
• Gene symbol: PDCD6
• Gene ID (NCBI): 10016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Programmed cell death protein 6 (PDCD6) is also named as ALG2. PDCD6 is an evolutionarily conserved Ca2+-binding protein. PDCD6 is required for coat protein complex II-dependent endoplasmic reticulum-to-Golgi apparatus vesicular transport in the cytoplasm (PMID: 39934130). PDCD6 is involved in regulating multifaceted and pleiotropic cellular processes in different cellular compartments. The nuclear PDCD6 regulates apoptosis and alternative splicing. And cytoplasmic PDCD6 is involved in the regulation of cytoskeletal dynamics and innate immune responses. Additionally, membranous PDCD6 participates in membrane repair through endosomal sorting complex required for transport complex-dependent membrane budding. (PMID: 38436472). PDCD6 also acts as a regulator of cytosolic DNA-induced immune responses by modulating STING transport (PMID: 34787301).