PDCD6 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDCD6 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

19

Kat-Nr. : 12303-1-AP

Synonyme

ALG 2, ALG2, PDCD6, PEF1B, programmed cell death 6

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Galerie der Validierungsdaten

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  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12303-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 antibody) at dilution of 1:2000 incubated at 4 degree celsius over night.

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  WB analysis of Jurkat using 12303-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 12303-1-AP (PDCD6 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 12303-1-AP

  IP Result of anti-PDCD6 (IP:12303-1-AP, 3ug; Detection:12303-1-AP 1:500) with HeLa cells lysate 3200ug.

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  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human stomach using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 12303-1-AP (PDCD6 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12303-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12303-1-AP (PDCD6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen, HeLa-Zellen, HepG2-Zellen, Jurkat-Zellen, K-562-Zellen, Mauslebergewebe, NIH/3T3-Zellen, Rattenlebergewebe
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: humanes Lungenkarzinomgewebe, humanes Leberkarzinomgewebe, humanes Magengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:8000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 5 publications below
• WB: See 15 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Produktinformation

12303-1-AP bindet in WB, IP, IHC, IF, ELISA PDCD6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDCD6 fusion protein Ag2949
• Vollständiger Name: programmed cell death 6
• Berechnetes Molekulargewicht: 191 aa, 22 kDa
• Beobachtetes Molekulargewicht: 15-22 kDa
• GenBank-Zugangsnummer: BC012384
• Gene symbol: PDCD6
• Gene ID (NCBI): 10016
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Nature; Structural basis for dimerization quality control.; Authors - Elijah L Mena
human	IF	Science; Triggered recruitment of ESCRT machinery promotes endolysosomal repair.; Authors - Michael L Skowyra
human	WB,IF	Cell; Regulation of the CUL3 Ubiquitin Ligase by a Calcium-Dependent Co-adaptor.; Authors - Colleen A McGourty ; KD Validated
human	WB	Mol Cell; Co-adaptor driven assembly of a CUL3 E3 ligase complex.; Authors - David Akopian
human	WB,IF	Cell Rep; Calcium-dependent ESCRT recruitment and lysosome exocytosis maintain epithelial integrity during Candida albicans invasion.; Authors - Johannes Westman ; KD Validated
human	IF	J Cell Biol; Giant worm-shaped ESCRT scaffolds surround actin-independent integrin clusters; Authors - Femmy C Stempels