PDCL2 Polyklonaler Antikörper

PDCL2 Polyklonal Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IF, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 17407-1-AP

Synonyme

GCPHLP, PDCL2, phosducin like 2, Phosducin like protein 2

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  WB analysis using 17407-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 17407-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 17407-1-AP (PDCL2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 17407-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17407-1-AP (PDCL2 antibody) at dilution of 1:50 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushodengewebe, Rattenhodengewebe
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Eierstockgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:4000
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

17407-1-AP bindet in WB, IF, IHC, ELISA PDCL2 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDCL2 fusion protein Ag11427
• Vollständiger Name: phosducin-like 2
• Berechnetes Molekulargewicht: 250 aa, 29 kDa
• Beobachtetes Molekulargewicht: 30-35 kDa
• GenBank-Zugangsnummer: BC034431
• Gene symbol: PDCL2
• Gene ID (NCBI): 132954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
mouse	WB,IF	Andrology; PDCL2 is essential for sperm acrosome formation and male fertility in mice; Authors - Yoshitaka Fujihara
human	IHC	Transl Androl Urol; Identification of PDCL2 as a candidate marker in Sertoli cell-only syndrome by chromatin immunoprecipitation-sequencing and bioinformatics analysis; Authors - Yu Yang
mouse	WB	PNAS Nexus; Tex46 knockout male mice are sterile secondary to sperm head malformations and failure to penetrate through the zona pellucida; Authors - Yoshitaka Fujihara