PDE10A Polyklonaler Antikörper

PDE10A Polyklonal Antikörper für IHC, IF/ICC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus und mehr (1)

Anwendung

WB, IHC, IF/ICC, IP, CoIP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 18078-1-AP

Synonyme

phosphodiesterase 10A, HSPDE10A, EC:3.1.4.17, cAMP and cAMP-inhibited cGMP 3',5'-cyclic phosphodiesterase 10A

---

• 

  IP experiment of mouse brain using 18078-1-AP

  IP result of anti-PDE10A (IP:18078-1-AP, 4ug; Detection:18078-1-AP 1:300) with mouse brain tissue lysate 5200ug.

• 

  IHC staining of mouse brain using 18078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18078-1-AP (PDE10A antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 18078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 18078-1-AP (PDE10A antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 18078-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PDE10A antibody (18078-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Geprüfte Anwendungen

• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF/ICC: HeLa-Zellen

Empfohlene Verdünnung

• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Produktinformation

18078-1-AP bindet in WB, IHC, IF/ICC, IP, CoIP, ELISA PDE10A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human, Maus, Rind
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE10A fusion protein Ag12771
• Vollständiger Name: phosphodiesterase 10A
• Berechnetes Molekulargewicht: 779 aa, 88 kDa
• Beobachtetes Molekulargewicht: 89 kDa
• GenBank-Zugangsnummer: BC104860
• Gene symbol: PDE10A
• Gene ID (NCBI): 10846
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE10A (Phosphodiesterase 10A) is a single member of the PDE10 protein family, and it catalyzes both cAMP and cGMP hydrolysis (PMID: 34550322). The literature on PDE10A has been focused on psychiatric and neurological disorders because PDE10A expression is enriched in the striatal region of the brain under normal conditions (PMID: 12967715). Moreover, PDE10A expression is induced in various human tumor cell lines and PDE10A inhibition suppresses tumor cell growth (PMID: 37232184).

Publikationen

Species	Application	Title
human,mouse	WB	Environ Int; Exposure to dithiocarbamate fungicide maneb in vitro and in vivo: Neuronal apoptosis and underlying mechanisms; Authors - Chaoyang Liu
human	WB	EMBO J; A genetically targeted reporter for PET imaging of deep neuronal circuits in mammalian brains.; Authors - Masafumi Shimojo
human	WB,IF	Cell Mol Life Sci; Crip2 affects vascular development by fine-tuning endothelial cell aggregation and proliferation; Authors - Shuaiqi Yang
bovine	WB	Andrology; Characterization of cAMP-phosphodiesterase activity in bovine seminal plasma.; Authors - A Bergeron
bovine	WB,IF	Andrology; Papaverine-sensitive phosphodiesterase activity is measured in bovine spermatozoa.; Authors - A Bergeron
human	WB,IHC,IF,CoIP	Biochem Pharmacol; N6-methyladenosine-modified RNF220 induces cisplatin resistance and immune escape via regulating PDE10A K48-linked ubiquitination in bladder cancer; Authors - Kai Li