PDE3B Polyklonaler Antikörper

PDE3B Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (2)

Anwendung

WB, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(3)

Kat-Nr. : 25290-1-AP

Synonyme

CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B

---

• 

  WB analysis of HL-60 using 25290-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 25290-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 25290-1-AP

  IP result of anti-PDE3B (IP:25290-1-AP, 5ug; Detection:25290-1-AP 1:300) with HeLa cells lysate 800ug.

---

Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HL-60-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 3 publications below

Produktinformation

25290-1-AP bindet in WB, IP, ELISA PDE3B und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE3B fusion protein Ag18064
• Vollständiger Name: phosphodiesterase 3B, cGMP-inhibited
• Berechnetes Molekulargewicht: 1112 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC136565
• Gene symbol: PDE3B
• Gene ID (NCBI): 5140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).

Publikationen

Species	Application	Title
mouse,human	WB	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu
human	WB	Mol Carcinog; miR-135b-5p/PDE3B Axis Regulates Gemcitabine Resistance in Pancreatic Cancer Through Epithelial-Mesenchymal Transition; Authors - Yuxuan Fu
rat	WB	Drug Des Devel Ther; The Mechanism of Xuanyu Tongjing Decoction Regulating NOD/NFκB Pathway to Inhibit Ectopic Tissue Inflammation to Reduce Ovarian Damage in Rats with Ovarian Endometriosis; Authors - Weisen Fan