PDE3B Rekombinanter Antikörper

PDE3B Rekombinant Antikörper für WB, IHC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

CloneNo.

250460H7

Kat-Nr. : 85996-1-RR

Synonyme

CGIP1, CGI-PDE B, cGIPDE1, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, Cyclic GMP-inhibited phosphodiesterase B

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  WB analysis using 85996-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse adipose using 85996-1-RR

  Mouse adipose tissue were subjected to SDS PAGE followed by western blot with 85996-1-RR (PDE3B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse adipose using 85996-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse adipose using 85996-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse adipose tissue slide using 85996-1-RR (PDE3B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 85996-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85996-1-RR against Human PDE3B were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, Jurkat-Zellen, Maus-Fettgewebe
• Erfolgreiche Detektion in IHC: Maus-Fettgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunhistochemie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

85996-1-RR bindet in WB, IHC, ELISA PDE3B und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDE3B fusion protein Ag19190
• Vollständiger Name: phosphodiesterase 3B, cGMP-inhibited
• Berechnetes Molekulargewicht: 1112 aa, 124 kDa
• Beobachtetes Molekulargewicht: 124 kDa
• GenBank-Zugangsnummer: BC136565
• Gene symbol: PDE3B
• Gene ID (NCBI): 5140
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE3B is a cyclic nucleotide phosphodiesterase with dual specificity for cAMP and cGMP. It plays crucial roles in regulating physiological processes such as angiogenesis and cardiac contractility. PDE3B can be activated by insulin and is involved in the antilipolytic actions of insulin. Additionally, PDE3B forms macromolecular complexes that are important for its activation and interaction with other proteins (PMID: 22001403, PMID: 26031333).