PDE4B Polyklonaler Antikörper

PDE4B Polyklonal Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 31730-1-PBS

Synonyme

DPDE4, PDE32, PDE4B5, PDEIVB

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  WB analysis using 31730-1-AP (same clone as 31730-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 31730-1-AP (PDE4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31730-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 31730-1-AP (same clone as 31730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31730-1-AP (PDE4B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 31730-1-AP (same clone as 31730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31730-1-AP (PDE4B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 31730-1-AP (same clone as 31730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 31730-1-AP (PDE4B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31730-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 31730-1-AP (same clone as 31730-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31730-1-AP (PDE4B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31730-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

31730-1-PBS bindet in WB, IHC, Indirect ELISA PDE4B und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE4B fusion protein Ag35552
• Vollständiger Name: phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
• Berechnetes Molekulargewicht: 736 aa, 83 kDa
• Beobachtetes Molekulargewicht: 83 kDa
• GenBank-Zugangsnummer: BC105040
• Gene symbol: PDE4B
• Gene ID (NCBI): 5142
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE4B, also known as DPDE4, is a member of the PDE family of type IV cAMP-specific cyclic nucleotides. PDE4B regulates the cellular concentration of cyclic nucleotides and thus plays a role in signal transduction of inflammatory factors. Among all subtypes of PDE4, PDE4B is closely associated with cancer and has a major contribution to the role in hematological malignancies. PDE4B is distributed in multiple tissues, especially myeloid cells (PMID: 35899614, PMID: 36278172).