PDE4D Monoklonaler Antikörper

PDE4D Monoklonal Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

Hausschwein, human, Maus, Ratte

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1C4B9

Kat-Nr. : 67062-1-PBS

Synonyme

1C4B9, 3',5'-cyclic-AMP phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D, DPDE3, EC:3.1.4.53

---

• 

  WB analysis using 67062-1-Ig (same clone as 67062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cerebellum using 67062-1-Ig (same clone as 67062-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67062-1-Ig (same clone as 67062-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67062-1-Ig (same clone as 67062-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67062-1-Ig (same clone as 67062-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67062-1-Ig (same clone as 67062-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDE4D antibody (67062-1-Ig, Clone: 1C4B9 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

67062-1-PBS bindet in WB, IF/ICC, Indirect ELISA PDE4D und zeigt Reaktivität mit Hausschwein, human, Maus, Ratten

• Getestete Reaktivität: Hausschwein, human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDE4D fusion protein Ag8302
• Vollständiger Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Berechnetes Molekulargewicht: 809 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91-110 kDa, 66-84 kDa
• GenBank-Zugangsnummer: BC036319
• Gene symbol: PDE4D
• Gene ID (NCBI): 5144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-G-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE4D(cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also known as DPDE3, PDE43 and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram (PMID:16730340). It has some isoforms and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in the hippocampus from a patient with advanced Alzheimer disease (PMID:17353396). Bands of 90-110 kDa correspond to the long PDE4D isoforms, and bands of 66-84 kDa are the short PDE4D isoforms (PMID: 17215491).