PDE4D Rekombinanter Antikörper

PDE4D Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

251427D3

Kat-Nr. : 86745-1-RR

Synonyme

3',5'-cyclic-AMP phosphodiesterase 4D, cAMP-specific phosphodiesterase 4D, DPDE3, EC:3.1.4.53, PDE43

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  WB analysis of mouse pancreas using 86745-1-RR

  Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 86745-1-RR

  Rat brain tissue were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 86745-1-RR

  K-562 cells were subjected to SDS PAGE followed by western blot with 86745-1-RR (PDE4D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 86745-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86745-1-RR against Human PDE4D were performed. The affinity constant is 3.51 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maus-Pankreasgewebe, K-562-Zellen, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

86745-1-RR bindet in WB, ELISA PDE4D und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDE4D fusion protein Ag3588
• Vollständiger Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Berechnetes Molekulargewicht: 809 aa, 91 kDa
• Beobachtetes Molekulargewicht: 91 kDa
• GenBank-Zugangsnummer: BC036319
• Gene symbol: PDE4D
• Gene ID (NCBI): 5144
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE4D (cAMP-specific 3',5'-cyclic phosphodiesterase 4D) is also named as DPDE3, PDE43, and belongs to the cyclic nucleotide phosphodiesterase family and PDE4 subfamily. PDE4D subtype may be of particular importance as an antidepressant target in that it is regulated by repeated treatment with both norepinephrine and serotonin reuptake inhibitors as well as by the PDE4 inhibitor rolipram(PMID:16730340). It has some isoforms, and all the PDE4D isoforms are expressed in healthy adult human hippocampus and in hippocampus from a patient with advanced Alzheimer's disease(PMID:17353396).