PDE5A Rekombinanter Antikörper

PDE5A Rekombinant Antikörper für WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242597B10

Kat-Nr. : 85030-1-PBS

Synonyme

242597B10, CGB-PDE, cGMP-binding cGMP-specific phosphodiesterase, cGMP-specific 3',5'-cyclic phosphodiesterase, CN5A

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  WB analysis of rat lung using 85030-1-RR (same clone as 85030-1-PBS)

  rat lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse lung using 85030-1-RR (same clone as 85030-1-PBS)

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 85030-1-RR (PDE5A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP01762-3

  The mean PDE5A concentration was determined to be 34.07 ng/mL in HeLa cell extract based on a 1.5 mg/mL extract load and 35.64 ng/mL in MCF-7 cell extract based on a 1.2 mg/mL extract load.

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  Sandwich ELISA standard curve of MP01762-3

  Sandwich ELISA standard curve of MP01762-3, Human PDE5A Recombinant Matched Antibody Pair - PBS only. 85030-4-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag18779. 85030-1-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

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  IHC staining of human ovary cancer using 85030-1-RR (same clone as 85030-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 85030-1-RR (PDE5A antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

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  IF Staining of SKOV-3 using 85030-1-RR (same clone as 85030-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDE5A antibody (85030-1-RR, Clone: 242597B10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

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  FC experiment of HEK-293 using 85030-1-RR (same clone as 85030-1-PBS)

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PDE5A Recombinant antibody (85030-1-RR, Clone:242597B10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 85030-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 85030-1-RR

  Biolayer interferometry (BLl) kinetic assays of 85030-1-RR against Human PDE5A were performed. The affinity constant is 1.07 nM.

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Geprüfte Anwendungen

Produktinformation

85030-1-PBS bindet in WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA PDE5A und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDE5A fusion protein Ag18779
• Vollständiger Name: phosphodiesterase 5A, cGMP-specific
• Berechnetes Molekulargewicht: 875 aa, 100 kDa
• Beobachtetes Molekulargewicht: 100 kDa
• GenBank-Zugangsnummer: BC126233
• Gene symbol: PDE5A
• Gene ID (NCBI): 8654
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE5A (cGMP-binding cGMP-specific phosphodiesterase) plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. This phosphodiesterase catalyzes the specific hydrolysis of cGMP to 5'-GMP. Through alternative splicing, the PDE5A gene produces three proteins (PDE5A1, PDE5A2, and PDE5A3) that differ in their N termini and range in mass from 89 to 105 kDa. (PMID: 25704994; 21215707). A second 70 kDa band was consistently observed in heart tissue that either reflected a splice variant or proteolytic fragment of PDE5A. (PMID: 15576651).