PDE8A Polyklonaler Antikörper

PDE8A Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13956-1-PBS

Synonyme

---

• 

  WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse spleen using 13956-1-AP (same clone as 13956-1-PBS)

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 13956-1-AP (same clone as 13956-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 13956-1-AP (PDE8A antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IP experiment of mouse testis using 13956-1-AP (same clone as 13956-1-PBS)

  IP result of anti-PDE8A (IP:13956-1-AP, 5ug; Detection:13956-1-AP 1:1000) with mouse testis tissue lysate 8000ug. This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon cancer using 13956-1-AP (same clone as 13956-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 13956-1-AP (PDE8A antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 13956-1-PBS in a different storage buffer formulation.

---

Geprüfte Anwendungen

Produktinformation

13956-1-PBS bindet in WB, IHC, IP, Indirect ELISA PDE8A und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDE8A fusion protein Ag5069
• Vollständiger Name: phosphodiesterase 8A
• Berechnetes Molekulargewicht: 93 kDa
• Beobachtetes Molekulargewicht: 93 kDa, 88 kDa
• GenBank-Zugangsnummer: BC060762
• Gene symbol: PDE8A
• Gene ID (NCBI): 5151
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDE8A(high affinity cAMP-specific and IBMX-insensitive 3',5'-cyclic phosphodiesterase 8A) belongs to the cyclic nucleotide phosphodiesterase family and PDE8 subfamily. PDE8A plays a critical role in the modulation of at least one compartment of cAMP and hence PKA activity during β-adrenergic receptor (βAR) activation in ventricular myocytes(PMID:20353794). The full length protein contains several sites for protein phosphorylation, N-glycosylation, N-myristoylation, and amidation. It has 5 isoforms produced by alternative splicing with the molecular mass of 93 kDa, 88 kDa, 51 kDa and the isoform 4 and 5 have the same molecular mass of 66 kDa.(PMID:11738832).