PDGFR beta/CD140b Rekombinanter Antikörper

PDGFR beta/CD140b Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241754A6

Kat-Nr. : 84388-5-RR

Synonyme

241754A6, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140b

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  WB analysis using 84388-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84388-5-RR (PDGF R beta antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of NIH/3T3 using 84388-5-RR

  Immunofluorescent analysis of (-20°C Methanol) fixed NIH/3T3 cells using PDGFR beta/CD140b antibody (84388-5-RR, Clone: 241754A6 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84388-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84388-5-RR against Mouse PDGF R beta were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: NIH/3T3-Zellen, C2C12-Zellen
• Erfolgreiche Detektion in IF/ICC: NIH/3T3-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84388-5-RR bindet in WB, IF/ICC, ELISA PDGFR beta/CD140b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDGFR beta/CD140b fusion protein Eg1533
• Vollständiger Name: platelet derived growth factor receptor, beta polypeptide
• Berechnetes Molekulargewicht: 123kDa
• Beobachtetes Molekulargewicht: 180 kDa
• GenBank-Zugangsnummer: NM_008809.2
• Gene symbol: PDGFR beta
• Gene ID (NCBI): 18596
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-derived growth Factor Receptor Beta (PDGFRβ) is a member of the receptor tyrosine kinase family of proteins, which plays a crucial role in various physiological and pathological conditions.