PDGFA Polyklonaler Antikörper

PDGFA Polyklonal Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 26940-1-AP

Synonyme

PDGF 1, PDGF A, PDGF AA, PDGF subunit A, PDGF1

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  WB analysis using 26940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Maushirngewebe, Mausnierengewebe, Rattenhirngewebe, Rattennierengewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

26940-1-AP bindet in WB, ELISA PDGFA und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDGFA fusion protein Ag25643
• Vollständiger Name: platelet-derived growth factor alpha polypeptide
• Berechnetes Molekulargewicht: 196 aa, 22 kDa
• Beobachtetes Molekulargewicht: 13-16 kDa
• GenBank-Zugangsnummer: BC146594
• Gene symbol: PDGFA
• Gene ID (NCBI): 5154
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDGFA is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFA plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFA is also involved in oligodendrocyte development and the myelination process in the spinal cord and cerebellum. PDGF-A protein monomer is about 13-16 kDa, and PDGF-AA is a 28.5 kDa A chain homodimer.