• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDGF-BB Rekombinanter Antikörper

PDGF-BB Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230486A7

Kat-Nr. : 83214-5-PBS

Synonyme

PDGFB, PDGFbb, PDGF2, PDGF subunit B, PDGF 2

Filter:
  • Western Blot (WB) analysis of various lysates using PDGF-BB Recombinant antibody (83214-5-RR)

    WB analysis using 83214-5-RR (same clone as 83214-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using PDGF-BB Recombinant antibody (83214-5-RR)

    WB analysis of A549 using 83214-5-RR (same clone as 83214-5-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83214-5-RR

    Affinity and Kinetic Characterization of 83214-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83214-5-RR against Human PDGFB were performed. The affinity constant is 16.9 nM.

Geprüfte Anwendungen

Produktinformation

83214-5-PBS bindet in WB, ELISA PDGF-BB und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PDGF-BB fusion protein Ag25986
Vollständiger Name platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
Berechnetes Molekulargewicht 241 aa, 27 kDa
Beobachtetes Molekulargewicht27 kDa
GenBank-ZugangsnummerBC029822
Gene symbol PDGFB
Gene ID (NCBI) 5155
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.