PDGF-BB Rekombinanter Antikörper

PDGF-BB Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

CloneNo.

230486A7

Kat-Nr. : 83214-5-RR

Synonyme

PDGFB, PDGFbb, PDGF2, PDGF subunit B, PDGF 2

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  WB analysis using 83214-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 83214-5-RR

  A549 cells were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 83214-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83214-5-RR against Human PDGFB were performed. The affinity constant is 16.9 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HUVEC-Zellen, A549-Zellen, Maushirngewebe, Rattenhirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

83214-5-RR bindet in WB, ELISA PDGF-BB und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDGF-BB fusion protein Ag25986
• Vollständiger Name: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• Berechnetes Molekulargewicht: 241 aa, 27 kDa
• Beobachtetes Molekulargewicht: 27 kDa
• GenBank-Zugangsnummer: BC029822
• Gene symbol: PDGFB
• Gene ID (NCBI): 5155
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.