PDGFC Polyklonaler Antikörper

PDGFC Polyklonal Antikörper für IHC, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, ELISA

Konjugation

Unkonjugiert

Publikationen

2

Kat-Nr. : 55076-1-AP

Synonyme

FALLOTEIN, PDGF C, PDGFC, PDGFC receptor binding form, SCDGF, VEGF E

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Galerie der Validierungsdaten

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  WB analysis of MDA-MB-453s using 55076-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 55076-1-AP (PDGFC Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human gliomas using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human thyroid cancer using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 55076-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 55076-1-AP (PDGFC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: MDA-MB-453s-Zellen
• Erfolgreiche Detektion in IHC: humanes Nierengewebe, humanes Gliomgewebe, humanes Kolongewebe, humanes Schilddrüsenkarzinomgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:200-1:1000
• Immunhistochemie (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 2 publications below

Produktinformation

55076-1-AP bindet in WB, IHC, ELISA PDGFC und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: Peptid
• Vollständiger Name: platelet derived growth factor C
• Berechnetes Molekulargewicht: 39 kDa
• Beobachtetes Molekulargewicht: 39 kDa
• GenBank-Zugangsnummer: NM_016205
• Gene symbol: PDGFC
• Gene ID (NCBI): 56034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: 0,1 M NaHCO3, 0,1M Glyzin, 0,02% Natriumazid und 50% Glyzerin, pH 7,3.
• Lagerungsbedingungen: Bei -20℃ lagern. Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDGFC is a member of the platelet-derived growth factor family. It is a monomeric protein secreted as a latent dimeric factor. Expression of PDGFC was highest in prostate, testis, and uterus, with weaker expression in spleen, thymus, and small intestine, and little expression in colon and peripheral blood leukocytes.

Publikationen

Species	Application	Title
human	WB	Br J Cancer; Glycometabolic reprogramming-mediated proangiogenic phenotype enhancement of cancer-associated fibroblasts in oral squamous cell carcinoma: role of PGC-1α/PFKFB3 axis.; Authors - Xiang Li
human	WB	Int J Biol Sci; Roquin2 suppresses breast cancer progression by inhibiting tumor angiogenesis via selectively destabilizing proangiogenic factors mRNA.; Authors - Meicen Zhou