PDGFR beta/CD140b Rekombinanter Antikörper

PDGFR beta/CD140b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

Maus

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

241754F6

Kat-Nr. : 98207-1-RR

Synonyme

241754F6, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140b

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  FC experiment of NIH/3T3 using 98207-1-RR

  1x10^6 NIH/3T3 cells (left) or mouse splenocytes (right) were surface stained with 0.25 ug Anti-Mouse PDGFR beta/CD140b Rabbit Recombinant Antibody (98207-1-RR, Clone: 241754F6) (red) or Rabbit IgG Isotype Control Recombinant Antibody (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  Affinity and Kinetic Characterization of 98207-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98207-1-RR against Mouse PDGFR beta/CD140b were performed. The affinity constant is below 1 pM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: NIH/3T3-Zellen

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98207-1-RR bindet in FC PDGFR beta/CD140b und zeigt Reaktivität mit Maus

• Getestete Reaktivität: Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDGFR beta/CD140b fusion protein Eg1533
• Vollständiger Name: platelet derived growth factor receptor, beta polypeptide
• Berechnetes Molekulargewicht: 123kDa
• GenBank-Zugangsnummer: NM_008809.2
• Gene symbol: PDGFR beta
• Gene ID (NCBI): 18596
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

The Platelet-Derived Growth Factor Receptor Beta (PDGFR beta, also known as CD140b) is a receptor tyrosine kinase that plays a crucial role in various cellular processes, including cell proliferation, migration, and survival. In mice, PDGFR beta is particularly significant due to its expression in pericytes, which are cells that reside on the walls of capillaries and play a vital role in maintaining vascular stability and integrity (PMID: 20738866). In the central nervous system (CNS) of mice, PDGFR beta is predominantly expressed in pericytes and vascular smooth muscle cells. It is involved in the formation and maintenance of the blood-brain barrier, as well as in the regulation of angiogenesis and vascular stability. Research in mouse models has shown that PDGFR beta signaling is crucial for tissue repair and functional recovery after stroke. Mice with disrupted PDGFR beta signaling exhibit delayed recovery and increased infarction volume following cerebral ischemia (PMID: 21952111).