PDGFR beta/CD140b Rekombinanter Antikörper

PDGFR beta/CD140b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242711A9

Kat-Nr. : 98405-2-PBS

Synonyme

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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  FC experiment of MG-63 using 98405-2-RR (same clone as 98405-2-PBS)

  1x10^6 MG-63 cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98405-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98405-2-RR (same clone as 98405-2-PBS)

  1x10^6 HEK-293T cells or PDGFR beta/CD140b transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98405-2-PBS in a different storage buffer formulation.

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  FC experiment of Transfected HEK-293T using 98405-2-RR (same clone as 98405-2-PBS)

  1x10^6 HEK-293T cells (left) or PDGFR beta/CD140b transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human PDGFRB Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98405-2-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

98405-2-PBS bindet in FC PDGFR beta/CD140b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDGFR beta/CD140b fusion protein Eg3715
• Vollständiger Name: platelet-derived growth factor receptor, beta polypeptide
• Berechnetes Molekulargewicht: 1106 aa, 124 kDa
• GenBank-Zugangsnummer: BC032224
• Gene symbol: PDGFR beta
• Gene ID (NCBI): 5159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)