PDGFR beta/CD140b Rekombinanter Antikörper

PDGFR beta/CD140b Rekombinant Antikörper für FC

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC

Konjugation

Unkonjugiert

CloneNo.

242711A9

Kat-Nr. : 98405-2-RR

Synonyme

PDGFRB, Beta platelet-derived growth factor receptor, Beta-type platelet-derived growth factor receptor, CD140 antigen-like family member B, CD140B

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  FC experiment of MG-63 using 98405-2-RR

  1x10^6 MG-63 cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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  FC experiment of Transfected HEK-293T using 98405-2-RR

  1x10^6 HEK-293T cells or PDGFR beta/CD140b transfected HEK-293T cells were surface stained with 0.25 ug Anti-Human PDGFR beta/CD140b Rabbit RecAb (98405-2-RR, Clone: 242711A9) and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

• 

  FC experiment of Transfected HEK-293T using 98405-2-RR

  1x10^6 HEK-293T cells (left) or PDGFR beta/CD140b transfected HEK-293T cells(right) were surface stained with 0.25 ug Anti-Human PDGFRB Rabbit RecAb (98405-2-RR, Clone: 242711A9) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in FC: MG-63 cells, Transfected HEK-293T cells

Empfohlene Verdünnung

Anwendung	Verdünnung
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

98405-2-RR bindet in FC PDGFR beta/CD140b und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDGFR beta/CD140b fusion protein Eg3715
• Vollständiger Name: platelet-derived growth factor receptor, beta polypeptide
• Berechnetes Molekulargewicht: 1106 aa, 124 kDa
• GenBank-Zugangsnummer: BC032224
• Gene symbol: PDGFR beta
• Gene ID (NCBI): 5159
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.09% sodium azide
• Lagerungsbedingungen: Store at 2 - 8°C. Stable for one year after shipment.

Hintergrundinformationen

PDGFRs are receptor tyrosine kinases with an extracellular ligand-binding domain and a cytoplasmic enzymatic domain. The signaling activity of PDGFRs is tightly regulated by an autoinhibitory allosteric conformation, and requires ligand-induced receptor dimerization at the cell surface to alleviate autoinhibition and achieve physiological activity (PMID: 21664579). PDGFR beta (platelet-derived growth factor receptor- β)/CD140b is a constitutive marker expressed by ASCs in the angiogenic behavior of human retinal endothelial cells (HREs). CD140b (PDGFRβ) plays a crucial role in controlling the intrinsic proliferation (PMID: 30976657)