PDGFR alpha/CD140a Rekombinanter Antikörper

PDGFR alpha/CD140a Rekombinant Antikörper für WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

CloneNo.

241658G6

Kat-Nr. : 84383-2-RR

Synonyme

PDGFRA, CD140a antigen, CD140A, CD140 antigen-like family member A, Alpha-type platelet-derived growth factor receptor

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  WB analysis using 84383-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84383-2-RR (PDGFRA antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84383-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84383-2-RR against Human PDGFR alpha/CD140a were performed. The affinity constant is 1.21 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 1 publications below

Produktinformation

84383-2-RR bindet in WB, ELISA PDGFR alpha/CD140a und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: platelet-derived growth factor receptor, alpha polypeptide
• Berechnetes Molekulargewicht: 123 kDa
• Beobachtetes Molekulargewicht: 170-190 kDa
• GenBank-Zugangsnummer: BC015186
• Gene symbol: PDGFR alpha
• Gene ID (NCBI): 5156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Publikationen

Species	Application	Title
human	WB	Stem Cells Int; Human Umbilical Cord-Mesenchymal Stem Cells Combined With Low Dosage Nintedanib Rather Than Using Alone Mitigates Pulmonary Fibrosis in Mice; Authors - Huijun Qiu