PDGFR alpha/CD140a Rekombinanter Antikörper

PDGFR alpha/CD140a Rekombinant Antikörper für FC, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

FC, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

241658H4

Kat-Nr. : 98141-1-PBS

Synonyme

PDGFRA, 241658H4, Alpha platelet-derived growth factor receptor, Alpha-type platelet-derived growth factor receptor, CD140 antigen-like family member A

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  Sandwich ELISA standard curve of MP01256-4

  Sandwich ELISA standard curve of MP01256-4, Human PDGFR alpha/CD140a Recombinant Matched Antibody Pair - PBS only. 84383-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard RP00003. 98141-1-PBS was HRP conjugated as the detection antibody. Range: 39.1-2500 pg/mL.

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  Sample test of MP01256-4

  Serum of six individual healthy human donors was measured. The PDGFR alpha/CD140a concentration of detected samples was determined to be 2,557.7 pg/mL with a range of 1,472.3-4,545.6 pg/mL.

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  Sample test of MP01256-4

  The mean PDGFR alpha/CD140a concentration was determined to be 2.6 ng/mL in SH-SY5Y cell extract based on a 1.0 mg/mL extract load and 7.0 ng/mL in human placenta tissue extract based on a 1.7 mg/mL extract load.

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  FC experiment of MG-63 using 98141-1-RR (same clone as 98141-1-PBS)

  1x10^6 MG-63 cells (left) or A-172 cells (right) were surface stained with 0.25 ug Anti-Human PDGFR alpha/CD140a Rabbit Recombinant Antibody (98141-1-RR, Clone: 241658H4) (red) or Isotype Control (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were not fixed. This data was developed using the same antibody clone with 98141-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 98141-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98141-1-RR against Human PDGFR alpha/CD140a were performed. The affinity constant is 2.26 nM.

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Geprüfte Anwendungen

Produktinformation

98141-1-PBS bindet in FC, Sandwich ELISA, Indirect ELISA, Sample test PDGFR alpha/CD140a und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: Rekombinantes Protein
• Vollständiger Name: platelet-derived growth factor receptor, alpha polypeptide
• Berechnetes Molekulargewicht: 123 kDa
• GenBank-Zugangsnummer: BC015186
• Gene symbol: PDGFR alpha
• Gene ID (NCBI): 5156
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein A purfication
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C.

Hintergrundinformationen

Platelet-derived growth factor receptor alpha (PDGFR alpha, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.