PDI Rekombinanter Antikörper

• Featured Product
• KD/KO Validated

PDI Rekombinant Antikörper für WB, IHC, IF-P, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF-P, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

4K17

Rezensionen (1)

Kat-Nr. : 80170-1-PBS

Synonyme

P4HB, 4K17, Cellular thyroid hormone-binding protein, CLCRP1, DSI

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  WB analysis using 80170-1-RR (same clone as 80170-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 80170-1-RR (PDI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 80170-1-RR (same clone as 80170-1-PBS)

  WB result of PDI antibody (80170-1-RR; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDI transfected HEK-293 cells. This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 80170-1-RR (same clone as 80170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 80170-1-RR (PDI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human prostate cancer using 80170-1-RR (same clone as 80170-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 80170-1-RR (PDI antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse lung using 80170-1-RR (same clone as 80170-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using PDI antibody (80170-1-RR, Clone: 4K17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  IF Staining of mouse lung using 80170-1-RR (same clone as 80170-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using PDI antibody (80170-1-RR, Clone: 4K17 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80170-1-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 80170-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80170-1-RR against Human PDI were performed. The affinity constant is 13.8 nM.

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Geprüfte Anwendungen

Produktinformation

80170-1-PBS bindet in WB, IHC, IF-P, Indirect ELISA PDI und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDI fusion protein Ag1747
• Vollständiger Name: prolyl 4-hydroxylase, beta polypeptide
• Berechnetes Molekulargewicht: 57 kDa
• Beobachtetes Molekulargewicht: 57 kDa
• GenBank-Zugangsnummer: BC014504
• Gene symbol: PDI
• Gene ID (NCBI): 5034
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA1(Protein disulfide-isomerase) is also named as ERBA2L, PDI, P4HB, PO4DB. It is a multifunctional protein that catalyzes the formation, breakage and rearrangement of disulfide bonds. In some cell types, it seems to be secreted or associated with the plasma membrane, where it undergoes constant shedding and replacement from intracellular sources.It can exsit as homodimer and monomers and homotetramers may also occur(PMID:12095988).

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Xuejian (Verified Customer) (07-23-2025)

After using this PBS-only antibody conjugated with metal in Hyperion, it's extremely good under Hyperion Image Mass Cytometry on mouse lung tissues.

• Applications: Other

PDI Antibody Other validation ( dilution) in (Cat no:80170-1-PBS)