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PDIA2 Rekombinanter Antikörper

PDIA2 Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242294E10

Kat-Nr. : 84765-4-PBS

Synonyme

242294E10, PDA2, PDI, PDIP, PDIR

Filter:
  • Western Blot (WB) analysis of various lysates using PDIA2 Recombinant antibody (84765-4-RR)

    WB analysis using 84765-4-RR (same clone as 84765-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84765-4-RR (PDIA2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84765-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PDIA2 Recombinant antibody (84765-4-RR)

    IHC staining of mouse pancreas using 84765-4-RR (same clone as 84765-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84765-4-RR (PDIA2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84765-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PDIA2 Recombinant antibody (84765-4-RR)

    IHC staining of mouse pancreas using 84765-4-RR (same clone as 84765-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84765-4-RR (PDIA2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84765-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84765-4-RR

    Affinity and Kinetic Characterization of 84765-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84765-4-RR against Human PDIA2 were performed. The affinity constant is 24.0 pM.

Geprüfte Anwendungen

Produktinformation

84765-4-PBS bindet in WB, IHC, Indirect ELISA PDIA2 und zeigt Reaktivität mit human, Maus, Ratten

Getestete Reaktivität human, Maus, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PDIA2 fusion protein Ag35301
Vollständiger Name protein disulfide isomerase family A, member 2
Berechnetes Molekulargewicht58 kDa
Beobachtetes Molekulargewicht60-65 kDa
GenBank-ZugangsnummerNM_006849.2
Gene symbol PDIA2
Gene ID (NCBI) 64714
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein A purfication
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA2 (Protein disulfide-isomerase A2), a member of PDI family, is an ER protein chaperone that catalyzes the formation and breakage of disulfide bonds between cysteine residues and can inhibit aggregation of misfolded proteins by catalyzing rearrangement of -S-S- bonds in proteins. (PMID: 23486466)