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PDIA4 Rekombinanter Antikörper

PDIA4 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250697E10

Kat-Nr. : 86239-1-PBS

Synonyme

ERp72, EC:5.3.4.1, ERp 72, ERp70, ERp-72

Filter:
  • Western Blot (WB) analysis of various lysates using PDIA4 Recombinant antibody (86239-1-RR)

    WB analysis using 86239-1-RR (same clone as 86239-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86239-1-RR (PDIA4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PDIA4 Recombinant antibody (86239-1-RR)

    IF Staining of HeLa using 86239-1-RR (same clone as 86239-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PDIA4 antibody (86239-1-RR, Clone: 250697E10 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 86239-1-PBS in a different storage buffer formulation.

Geprüfte Anwendungen

Produktinformation

86239-1-PBS bindet in WB, IF/ICC, Indirect ELISA PDIA4 und zeigt Reaktivität mit human, Maus

Getestete Reaktivität human, Maus
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PDIA4 fusion protein Eg3011
Vollständiger Name protein disulfide isomerase family A, member 4
Berechnetes Molekulargewicht73 kDa
Beobachtetes Molekulargewicht75 kDa
GenBank-ZugangsnummerNM_004911.5
Gene symbol ERp72
Gene ID (NCBI) 9601
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.