PDIR Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

PDIR Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(6)

Kat-Nr. : 15545-1-AP

Synonyme

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

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  WB analysis of mouse liver using 15545-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15545-1-AP (PDIR antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 15545-1-AP

  IP result of anti-PDIR (IP:15545-1-AP, 4ug; Detection:15545-1-AP 1:1000) with mouse liver tissue lysate 6000 ug.

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  IHC staining of human placenta using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 15545-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15545-1-AP (PDIR antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Mauslebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Milzgewebe, humanes Hodengewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:3000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Produktinformation

15545-1-AP bindet in WB, IHC, IF, IP, ELISA PDIR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDIR fusion protein Ag7901
• Vollständiger Name: protein disulfide isomerase family A, member 5
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC001625
• Gene symbol: PDIR
• Gene ID (NCBI): 10954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Publikationen

Species	Application	Title
human	WB	Int J Mol Sci; Quantitative Proteomics and Differential Protein Abundance Analysis after the Depletion of PEX3 from Human Cells Identifies Additional Aspects of Protein Targeting to the ER.; Authors - Richard Zimmermann
human	WB,IHC	Oncotarget; Expression of protein disulfide isomerase family members correlates with tumor progression and patient survival in ovarian cancer.; Authors - Soma Samanta
human	WB	Viruses; PDIA4 Is a Host Factor Important for Lymphocytic Choriomeningitis Virus Infection; Authors - Mengwei Xu ; KD Validated
human	WB	FEBS J; Identification of signal peptide features for substrate specificity in human Sec62/Sec63-dependent ER protein import.; Authors - Stefan Schorr
mouse	WB	JCI Insight; Protein disulfide isomerase is essential for spermatogenesis in mice; Authors - Yaqiong Zhang
human	WB,IF	Int J Biol Sci; RUNX1-PDIA5 Axis Promotes Malignant Progression of Glioblastoma by Regulating CCAR1 Protein Expression; Authors - Qiankun Ji ; KD Validated