PDIR Polyklonaler Antikörper

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• KD/KO Validated

PDIR Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF, IP, ELISA

Konjugation

Unkonjugiert

Publikationen(1)

Kat-Nr. : 29593-1-AP

Synonyme

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

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  WB analysis using 29593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29593-1-AP (PDIR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis using 29593-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29593-1-AP (PDIR antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 29593-1-AP

  IP result of anti-PDIR (IP:29593-1-AP, 4ug; Detection:29593-1-AP 1:5000) with mouse liver tissue lysate 2400 ug.

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  IHC staining of human placenta using 29593-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29593-1-AP (PDIR antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 29593-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 29593-1-AP (PDIR antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HeLa-Zellen, HepG2-Zellen, L02-Zellen, Mauslebergewebe, Rattenlebergewebe
• Erfolgreiche IP: Mauslebergewebe
• Erfolgreiche Detektion in IHC: humanes Plazenta-Gewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:16000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Produktinformation

29593-1-AP bindet in WB, IHC, IF, IP, ELISA PDIR und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDIR fusion protein Ag31479
• Vollständiger Name: protein disulfide isomerase family A, member 5
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC001625
• Gene symbol: PDIR
• Gene ID (NCBI): 10954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIR(Protein disulfide isomerase-related protein), also known as PDIA5 (Protein Disulfide Isomerase Family A Member 5), is a member of the PDI gene family and also exhibits chaperone-like activity. PDIR protein is correlated with immune infiltration, which is highly expressed in glioma and participates in glioma progression(PMID: 33664747). PDIR contributes to disulfide bond rearrangement in ATF6α under stress conditions that necessary for ATF6α activation upon ER stress(PMID: 24636989).

Publikationen

Species	Application	Title
human	WB,IF	Front Immunol; Pan-Cancer Analysis of the Immunological Role of PDIA5: A Potential Target for Immunotherapy; Authors - Yu Chen ; KD Validated