PDIA6 Polyklonaler Antikörper

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• KD/KO Validated

PDIA6 Polyklonal Antikörper für IF, IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

17

Kat-Nr. : 18233-1-AP

Synonyme

ER protein 5, ERP5, P5, PDIA6, Protein disulfide isomerase A6, Protein disulfide isomerase P5, TXNDC7

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Galerie der Validierungsdaten

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  WB analysis using 18233-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 18233-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 18233-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18233-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 18233-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 18233-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 18233-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18233-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 18233-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18233-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18233-1-AP (PDIA6 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 18233-1-AP

  IP Result of anti-PDIA6 (IP:18233-1-AP, 4ug; Detection:18233-1-AP 1:3000) with HEK-293 cells lysate 3000ug.

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  IHC staining of human stomach using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human stomach using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human stomach cancer using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human spleen using 18233-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 18233-1-AP

  Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 18233-1-AP

  Immunofluorescent analysis of HeLa cells using 18233-1-AP (PDIA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: Rattennierengewebe, HEK-293-Zellen, HeLa-Zellen, L02-Zellen, Mausherzgewebe, Mauslebergewebe, Mauslungengewebe, Mausnierengewebe
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IHC: humanes Magengewebe, humanes Magenkrebsgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.
• Erfolgreiche Detektion in IF: HeLa-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:1000-1:4000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:20-1:200
• Immunfluoreszenz (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 16 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Produktinformation

18233-1-AP bindet in WB, IP, IHC, IF, ELISA PDIA6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDIA6 fusion protein Ag12940
• Vollständiger Name: protein disulfide isomerase family A, member 6
• Berechnetes Molekulargewicht: 48 kDa, 54 kDa
• Beobachtetes Molekulargewicht: 48-54 kDa
• GenBank-Zugangsnummer: BC001312
• Gene symbol: PDIA6
• Gene ID (NCBI): 10130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA6(Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family.It is one of the endoplasmic reticulum (ER) resident proteins that catalyze formation, reduction, and isomerization of disulfide bonds in proteins and are thought to play a role in folding of disulfide-bonded proteins(PMID:7590364).It has 2 isoforms produced by alternative splicing.This antibody is specific to PDIA6.

Publikationen

Species	Application	Title
human	IHC	Cancer Cell; p53 Is a Master Regulator of Proteostasis in SMARCB1-Deficient Malignant Rhabdoid Tumors.; Authors - Alessandro Carugo
human	WB	Cell Res; Comparison of viral RNA-host protein interactomes across pathogenic RNA viruses informs rapid antiviral drug discovery for SARS-CoV-2.; Authors - Shaojun Zhang
mouse	WB	J Exp Med; Essential cell-extrinsic requirement for PDIA6 in lymphoid and myeloid development.; Authors - Jin Huk Choi
human	WB	Nucleic Acids Res; Melanoma RBPome identification reveals PDIA6 as an unconventional RNA-binding protein involved in metastasis.; Authors - Neus Mestre-Farràs ; KD Validated
human	WB	J Immunother Cancer; MUC1-C is a master regulator of MICA/B NKG2D ligand and exosome secretion in human cancer cells; Authors - Yoshihiro Morimoto
human	WB,IF,IP	Antiviral Res; Venezuelan equine encephalitis virus E1 protein interacts with PDIA6 and PDI inhibition reduces alphavirus production; Authors - Lauren Panny