PDIA6 Monoklonaler Antikörper

PDIA6 Monoklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Maus / IgG1

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

1G2D4

Kat-Nr. : 66669-1-PBS

Synonyme

EC:5.3.4.1, ER protein 5, ERp5, P5, Protein disulfide isomerase A6

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  WB analysis using 66669-1-Ig (same clone as 66669-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66669-1-Ig (PDIA6 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver cancer using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66669-1-Ig (PDIA6 antibody) at dilution of 1:300 (under 10x lens). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66669-1-Ig (same clone as 66669-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using PDIA6 antibody (66669-1-Ig, Clone: 1G2D4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66669-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

66669-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PDIA6 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Maus / IgG1
• Klonalität: Monoklonal
• Typ: Antikörper
• Immunogen: PDIA6 fusion protein Ag27608
• Vollständiger Name: protein disulfide isomerase family A, member 6
• Berechnetes Molekulargewicht: 48 kDa, 54 kDa
• Beobachtetes Molekulargewicht: 54 kDa
• GenBank-Zugangsnummer: BC001312
• Gene symbol: PDIA6
• Gene ID (NCBI): 10130
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIA6 (Protein disulfide-isomerase A6) is also named as ERP5, P5, TXNDC7 and belongs to the protein disulfide isomerase family. It is one of the endoplasmic reticulum (ER) resident proteins that catalyze the formation, reduction, and isomerization of disulfide bonds in proteins and is thought to play a role in the folding of disulfide-bonded proteins (PMID:7590364). It has 2 isoforms produced by alternative splicing. This antibody is specific to PDIA6.