PDIR Rekombinanter Antikörper

PDIR Rekombinant Antikörper für WB, IHC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus

Anwendung

WB, IHC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

242971D11

Kat-Nr. : 85599-4-PBS

Synonyme

PDIA5, EC:5.3.4.1, Protein disulfide isomerase A5, Protein disulfide isomerase-related protein, Protein disulfide-isomerase A5

---

• 

  WB analysis using 85599-4-RR (same clone as 85599-4-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85599-4-RR (PDIA5 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85599-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 85599-4-RR (same clone as 85599-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85599-4-RR (PDIR antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85599-4-PBS in a different storage buffer formulation.

• 

  IHC staining of human placenta using 85599-4-RR (same clone as 85599-4-PBS)

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 85599-4-RR (PDIR antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85599-4-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 85599-4-RR

  Biolayer interferometry (BLl) kinetic assays of 85599-4-RR against Human PDIR were performed. The affinity constant is below 1 pM.

---

Geprüfte Anwendungen

Produktinformation

85599-4-PBS bindet in WB, IHC, Indirect ELISA PDIR und zeigt Reaktivität mit human, Maus

• Getestete Reaktivität: human, Maus
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDIR fusion protein Ag31479
• Vollständiger Name: protein disulfide isomerase family A, member 5
• Berechnetes Molekulargewicht: 60 kDa
• Beobachtetes Molekulargewicht: 60 kDa
• GenBank-Zugangsnummer: BC001625
• Gene symbol: PDIR
• Gene ID (NCBI): 10954
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDIR(Protein disulfide isomerase-related protein), also known as PDIA5 (Protein Disulfide Isomerase Family A Member 5), is a member of the PDI gene family and also exhibits chaperone-like activity. PDIR protein is correlated with immune infiltration, which is highly expressed in glioma and participates in glioma progression(PMID: 33664747). PDIR contributes to disulfide bond rearrangement in ATF6α under stress conditions that necessary for ATF6α activation upon ER stress(PMID: 24636989).