PDK1 Polyklonaler Antikörper

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• KD/KO Validated

PDK1 Polyklonal Antikörper für WB, IHC, IP, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IP, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18262-1-PBS

Synonyme

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, EC:2.7.11.2, PDH kinase 1, PDHK1

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  WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 18262-1-AP (same clone as 18262-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18262-1-AP (PDK1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IP experiment of SiHa using 18262-1-AP (same clone as 18262-1-PBS)

  IP result of anti-PDK1 (IP:18262-1-AP, 4ug; Detection:18262-1-AP 1:3000) with SiHa cells lysate 1520 ug. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat kidney using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 18262-1-AP (PDK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 18262-1-AP (same clone as 18262-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart slide using 18262-1-AP (PDK1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 18262-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18262-1-PBS bindet in WB, IHC, IP, Indirect ELISA PDK1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDK1 fusion protein Ag13121
• Vollständiger Name: pyruvate dehydrogenase kinase, isozyme 1
• Berechnetes Molekulargewicht: 436 aa, 49 kDa
• Beobachtetes Molekulargewicht: 46-55 kDa
• GenBank-Zugangsnummer: BC039158
• Gene symbol: PDK1
• Gene ID (NCBI): 5163
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDK1(pyruvate dehydrogenase kinase isoform 1) is also named as PDHK1 and belongs to the PDK/BCKDK protein kinase family. It inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism (PMID:17683942). Starvation increases islet PDK activity (PMID:11723055). PDK1 has 2 isoforms with the MW of 49 and 52 kD, and a 28aa-transit-peptide in the N-terminal can be removed in the mature form. This antibody is specific to PDK1.