PDK4 Polyklonaler Antikörper

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• KD/KO Validated

PDK4 Polyklonal Antikörper für WB, IHC, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IHC, IF/ICC, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 12949-1-PBS

Synonyme

PDHK4, EC:2.7.11.2, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial

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  WB analysis using 12949-1-AP (same clone as 12949-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  WB analysis of NCI-H1299 using 12949-1-AP (same clone as 12949-1-PBS)

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 12949-1-AP (PDK4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse heart using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse skeletal muscle using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat heart using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human pancreas cancer using 12949-1-AP (same clone as 12949-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12949-1-AP (PDK4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12949-1-AP (same clone as 12949-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PDK4 antibody (12949-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 12949-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

12949-1-PBS bindet in WB, IHC, IF/ICC, ELISA PDK4 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDK4 fusion protein Ag3629
• Vollständiger Name: pyruvate dehydrogenase kinase, isozyme 4
• Berechnetes Molekulargewicht: 411 aa, 46 kDa
• Beobachtetes Molekulargewicht: 46 kDa
• GenBank-Zugangsnummer: BC040239
• Gene symbol: PDK4
• Gene ID (NCBI): 5166
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Pyruvate dehydrogenase kinase isoform4 (PDK4) is also named as PDHK4 and belongs to the PDK/BCKDK protein kinase family. It is upregulated by starvation in many tissues of the body during starvation. This causes inactivation of the pyruvate dehydrogenase complex which blocks pyruvate oxidation and conserves lactate and alanine for gluconeogenesis. Enhanced PDK4 expression may be caused by the increase in free fatty acids that occurs during starvation. Free fatty acids can activate peroxisome proliferator-activated receptor α (PPARα), and activation of PPARα can promote PDK4 expression (PMID: 11554740).