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PDK4 Rekombinanter Antikörper

PDK4 Rekombinant Antikörper für Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Konjugation

Unkonjugiert

CloneNo.

240551F1

Kat-Nr. : 83583-3-PBS

Synonyme

[Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 4, mitochondrial, EC:2.7.11.2, PDHK4

Filter:
  • Sandwich ELISA standard curve of MP00556-3

    Sandwich ELISA standard curve of MP00556-3

    Sandwich ELISA standard curve of MP00556-3, Human PDK4 Recombinant Matched Antibody Pair - PBS only. 83583-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag3629. 83583-1-PBS was HRP conjugated as the detection antibody. Range: 0.47-30 ng/mL

  • Sample test of MP00556-3

    Sample test of MP00556-3

    Dissect the rat heart tissue and wash briefly with chilled 1X PBS to remove any blood if necessary, cut the tissue into smaller pieces whilst keeping it on ice. Transfer the tissue to a homogenizer and add Extraction Reagent with protease inhibitor. In general, add 500 μL Extraction Reagent for approximately every 10 mg of tissue. Homogenize thoroughly and keep the sample on ice for 30 min. Sonicate the sample and centrifuge at 10,000 x g, then transfer the supernatant to assay. PDK4 in rat heart tissue mesured 38.41 ng/ml total protein.

Geprüfte Anwendungen

Produktinformation

83583-3-PBS bindet in Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test PDK4 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PDK4 fusion protein Ag3629
Vollständiger Name pyruvate dehydrogenase kinase, isozyme 4
Berechnetes Molekulargewicht 411 aa, 46 kDa
GenBank-ZugangsnummerBC040239
Gene symbol PDK4
Gene ID (NCBI) 5166
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.