PDLIM4 Polyklonaler Antikörper

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PDLIM4 Polyklonal Antikörper für WB, IHC, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 18543-1-PBS

Synonyme

PDZ and LIM domain 4, Reversion-induced LIM protein, RIL

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  WB analysis using 18543-1-AP (same clone as 18543-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 18543-1-AP (PDLIM4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

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  WB analysis of A431 using 18543-1-AP (same clone as 18543-1-PBS)

  WB result of PDLIM4 antibody (18543-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM4 transfected A431 cells. This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

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  IHC staining of human skin cancer using 18543-1-AP (same clone as 18543-1-PBS)

  Immunohistochemical analysis of paraffin-embedded skin cancer slide using 18543-1-AP (PDLIM4 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

• 

  IF Staining of U2OS using 18543-1-AP (same clone as 18543-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PDLIM4 antibody (18543-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 18543-1-PBS in a different storage buffer formulation.

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Geprüfte Anwendungen

Produktinformation

18543-1-PBS bindet in WB, IHC, IF/ICC, Indirect ELISA PDLIM4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDLIM4 fusion protein Ag13424
• Vollständiger Name: PDZ and LIM domain 4
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC016765
• Gene symbol: PDLIM4
• Gene ID (NCBI): 8572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS only
• Lagerungsbedingungen: Store at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID: 12908099).