PDLIM4 Rekombinanter Antikörper

PDLIM4 Rekombinant Antikörper für WB, IF/ICC, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, ELISA

Konjugation

Unkonjugiert

CloneNo.

241401B3

Kat-Nr. : 84117-1-RR

Synonyme

RIL, Reversion-induced LIM protein, PDZ and LIM domain 4, 241401B3

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  WB analysis using 84117-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84117-1-RR (PDLIM4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of U2OS using 84117-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of U2OS using 84117-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PDLIM4 antibody (84117-1-RR, Clone: 241401B3 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  Affinity and Kinetic Characterization of 84117-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84117-1-RR against Human PDLIM4 were performed. The affinity constant is 2.43 nM.

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: A431-Zellen, K-562-Zellen, PC-3-Zellen
• Erfolgreiche Detektion in IF/ICC: U2OS-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:5000-1:50000
• Immunfluoreszenz (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

84117-1-RR bindet in WB, IF/ICC, ELISA PDLIM4 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Rekombinant
• Typ: Antikörper
• Immunogen: PDLIM4 fusion protein Ag13424
• Vollständiger Name: PDZ and LIM domain 4
• Berechnetes Molekulargewicht: 35 kDa
• Beobachtetes Molekulargewicht: 35 kDa
• GenBank-Zugangsnummer: BC016765
• Gene symbol: PDLIM4
• Gene ID (NCBI): 8572
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Protein-A-Reinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM4, also known as RIL, is a member of the ALP/Enigma family. PDLIM4 contains one PDZ(DHR) domain and one LIM zinc-binding domain. The LIM zinc-binding domain interacts with the second and fourth PDZ domains of Fap-1, possibly resulting in the dephosphorylation of PDLIM4 (PMID: 21636573). PDLIM4 is involved in osteoblast development and function. The variation of the PDLIM4 locus may be an important determinant of osteoporosis (PMID:12908099).