PDLIM7 Polyklonaler Antikörper

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• KD/KO Validated

PDLIM7 Polyklonal Antikörper für WB, IHC, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IHC, IP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 17080-1-AP

Synonyme

ENIGMA, LIM mineralization protein, LMP1

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  WB analysis of HeLa using 17080-1-AP

  WB result of PDLIM7 antibody (17080-1-AP; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDLIM7 transfected HeLa cells.

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  WB analysis using 17080-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17080-1-AP (PDLIM7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17080-1-AP

  IP result of anti-PDLIM7 (IP:17080-1-AP, 4ug; Detection:17080-1-AP 1:5000) with HeLa cells lysate 1200 ug.

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  IHC staining of mouse skeletal muscle using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human colon using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 17080-1-AP (PDLIM7 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human liver using 17080-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17080-1-AP (PDLIM7 antibody) at dilution of 1:100 (under 10x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: K-562-Zellen, HeLa-Zellen
• Erfolgreiche IP: HeLa-Zellen
• Erfolgreiche Detektion in IHC: Maus-Skelettmuskelgewebe, humanes Nierengewebe, humanes Lebergewebe, humanes Eierstockgewebe, humanes Hautgewebe, humanes Milzgewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:2000-1:10000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Produktinformation

17080-1-AP bindet in WB, IHC, IP, ELISA PDLIM7 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: PDLIM7 fusion protein Ag1334
• Vollständiger Name: PDZ and LIM domain 7 (enigma)
• Berechnetes Molekulargewicht: 50 kDa
• Beobachtetes Molekulargewicht: 48-50 kDa
• GenBank-Zugangsnummer: BC014521
• Gene symbol: PDLIM7
• Gene ID (NCBI): 9260
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS with 0.02% sodium azide and 50% glycerol
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.