• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PDLIM7 Rekombinanter Antikörper

PDLIM7 Rekombinant Antikörper für WB, IF/ICC, Indirect ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IF/ICC, Indirect ELISA

Konjugation

Unkonjugiert

CloneNo.

250775F12

Kat-Nr. : 86283-1-PBS

Synonyme

ENIGMA, LIM mineralization protein, LMP1

Filter:
  • Western Blot (WB) analysis of various lysates using PDLIM7 Recombinant antibody (86283-1-RR)

    WB analysis using 86283-1-RR (same clone as 86283-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86283-1-RR (PDLIM7,LMP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86283-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of SKOV-3 cells using PDLIM7 Recombinant antibody (86283-1-RR)

    IF Staining of SKOV-3 using 86283-1-RR (same clone as 86283-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed SKOV-3 cells using PDLIM7 antibody (86283-1-RR, Clone: 250775F12 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86283-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 86283-1-RR

    Affinity and Kinetic Characterization of 86283-1-RR

    Biolayer interferometry (BLl) kinetic assays of 86283-1-RR against Human PDLIM7 were performed. The affinity constant is 8.36 nM.

Geprüfte Anwendungen

Produktinformation

86283-1-PBS bindet in WB, IF/ICC, Indirect ELISA PDLIM7 und zeigt Reaktivität mit human

Getestete Reaktivität human
Wirt / Isotyp Kaninchen / IgG
Klonalität Rekombinant
Typ Antikörper
Immunogen PDLIM7 fusion protein Ag4614
Vollständiger Name PDZ and LIM domain 7 (enigma)
Berechnetes Molekulargewicht 55/60 kDa
Beobachtetes Molekulargewicht 50 kDa
GenBank-ZugangsnummerBC001093
Gene symbol PDLIM7
Gene ID (NCBI) 9260
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Protein-A-Reinigung
Lagerungspuffer PBS only
LagerungsbedingungenStore at -80°C. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

PDLIM7 (PDZ and LIM domain protein 7), also known as ENIGMA or LIM mineralization protein 1 (LMP-1), is a member of PDZ-LIM proteins which function as scaffold to interact with multiple proteins through N-terminal PDZ domain and C-terminal LIM domain. Its LIM domains bind to proteins involved in mitogenic signaling, and it plays a role in tumorigenesis by promoting cancer cell invasion and metastasis. In addition, PDLIM7 is an essential positive regulator of osteoblast differentiation and maturation and bone formation.